Isolation and Characterization of Lactic Acid Bacteria (Lab) from Small Intestine Content of Duck (Anas Sp.) As A Probiotic Candidate

Abstract

A group of lactic acid bacteria (LAB) as a probiotic agent have been known to have a beneficial effect on health chicken. The objectives of this study were to isolate, characterize and determine of the LAB which originated from the small intestine content of domestic duck (Anas sp.) for candidate of probiotic. Isolation, selection and identification of bacteria was done using de Man Rogosa Sharpe (MRS) agar + 1% bromocresol purple. Characterization of bacteria based on morphological by Gram staining methods, and identification of biochemical characters using catalase activity, and Triple Sugar Iron Agar (TSIA). The data of isolate confirmed to Bergey's Manual of Systematic Bacteriology. This research carried out on the Microbiology Laboratory, Microbiology Study Program, Yala Radjabhat University (YRU), Faculty of Science and Agriculture. The results showed that according to physiological and biochemical characteristics in various media, the bacteria had Gram +, catalase-, coccus in shape, and were able to ferment glucose to be lactic acid. Based on the result, it is concluded that the LAB isolate shows a similar characteristic to the member of genus Lactobacillus sp. The isolated bacteria shows characteristic resistance to acidic pH and bile salts so the bacteria were fulfilled as a probiotic candidate.