Influence of pH, temperature and dissolved oxygen concentration on the production of glucose 6-phosphate dehydrogenase and invertase by Saccharomyces cerevisiae

Abstract

The effect of pH (from 4.0 to 5.0), temperature (T) (from 30 °C to 40 °C) and dissolved oxygen concentration (DO) (from 0.2 to 6.0 mg O2/L) on glucose 6-phosphate dehydrogenase (G6PDH) (EC 1.1.1.49) and Invertase (EC 3.2.1.26) formation by S. cerevisiae were studied. The best culture conditions for G6PDH and Invertase formation were: 2.55 L culture medium (yeast extract, 3.0 g/L; 5peptone, 5.0 g/L; glucose, 2.0 g/L; sucrose, 15.0 g/L; Na2HPO4.12 H20, 2.4 g/L; (NH4)2SO4, 5.1 g/L and MgSO4 7H2O, 0.075 g/L); 0.45 L inoculum (0.70 g dry cell/L); pH = 4.5; T = 35 °C and DO = 4.0 mg/L. G6PDH was highly sensitive to pH, T and DO variation. The increase in G6PDH production was about three times when the DO ranged from 0.2 to 4.0 mg O2/L. Moreover, by shifting pH from 5.0 to 4.5 and temperature from 30 °C to 35 °C, G6PDH formation increased by 57% and 70%, respectively. Invertase activity (IA) of whole cells decreased at least 50% at extremes values of DO (2.0 and 6.0 mg O2/L) and pH (4.0 and 5.0). Furthermore, IA oscillated during the fermentation due to the glucose repression/derepression mechanism.