Chemical Composition of Bio-oil Produced by Hydrothermal Liquefaction of Microalgae with Different Lipid Content

Abstract

Bio-oil samples were obtained by hydrothermal liquefaction at 300 °C of three different stains of microalgae that differ by the bimolecular composition: Chlamydomonas, Chlamydopodium and Arthrospira. For bio-oil characterization the ultrahigh resolution Fourier transform ion cyclotron resonance mass spectrometry (FT ICR MS) was used. The results of the study showed that the number of reliably identified molecular formulas after filtration and deisotoping were 2794 (Chlamydomonas), 1514 (Chlamydopodium) and 2110 (Arthrospira) and 853 formulas in each sample were the common. At the same time the compounds presented in the bio-oil produced from microalgae with high content of lipids and hydrocarbons (Chlamydomonas and Chlamydopodium) were more saturated then the compounds in bio-oil produced from Arthrospira. In the bio-oil produced from all microalgae the compounds containing 1 and 2 nitrogen atoms dominate, and also ON2, N3, ON2 classes are present with relatively less intensity.