Using Daphnia magna Straus to monitor toxicity of sustainable oil sludge bio-phytoremediation

Abstract

Oil sludge (OS) is the toxic product of various oil refinery processes. Oil sludge biophytoremediation is sustainable remediation efforts were conducted to reduce OS toxicity level and they also need to be monitored through toxicity test. In this study, the toxicants were OS postbiophytoremediation for 24 months (OS24) and 40 months (OS40), and were tested to Daphnia magna. The toxicity test consisted of Range Finding Test, acute and chronic toxicity test. The purpose of this research is to obtain the LC50 value of each OS and the chronic effects shown by D. magna. The method in this study was experimental with Completely Randomized Design (CRD). The LC50-96 hours values were calculated from 50% of dead individuals while D. magna reproduction was calculated from the number of neonates (offspring) produced within three weeks. The LC50 of OS24 and OS40 were 0.042 ppm and 0.366 ppm respectively. Both were categorized as 'extremely toxic' refer to Swan, 1994, observed chronic effects were reproduction delays and a decrease in the number of neonates.