Antioxidant Activity of TEMON (Clitoria ternatea and Citrus sp.) as an Infused Herbal Tea

Abstract

Many people around the world believed that herbal tea had a lot of antioxidants contained in it, thus it was widely drunk all over the world. To increase the taste aromatic, color appearance, we combine taste and color, resulted tea with new color and taste and have high antioxidant. This research was conducted to evaluate antioxidant activities of butterfly flower or kembang telang (Clitoria ternatea), lemon (Citrus sp), combination of telang and lemon called TeMon. This study examines the antioxidant activity of herbal tea made from dried lemon (Citrus sp.) and telang (C. ternatea), telang tea, lemon tea. The antioxidant potential was assessed by performing on 2,2-diphenyl-1picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging activity assay, total phenolic and flavonoid content assay, 2,2-Azinobis 3-ethyl benzothiazoline 6-sulfonic acid (ABTS•+) reduction, and Ferric Reducing Antioxidant Power (FRAP) assay. Dried tea namely telang tea of 5 flower bud, lemon tea 5 g and mix of 5 flower bud telang and lemon 2 g (TeMon) were soaked in 200 ml hot water for 5 min resulted telang infusion, lemon infusion and TeMon infusion. All samples telang infusion, lemon infusion and TeMon infusion were assayed the antioxidant activity including scavenging activity of DPPH, H2O2, ABTS, FRAP assay and phenolic, flavonoid content. The median inhibitory concentration (IC50) was used to measure the antioxidant activity. The IC50 value of DPPH scavenging activity of telang tea, lemon tea, TeMon tea were 17.07%, 25.34%, 28.66 % respectively. The IC50 value for H2O2 scavenging activity respectively were 26.62%, 38.60%, 15.98%. The IC50 value of ABTS scavenging activity respectively were 2.51%, 3.50%, 3.27%. The IC50 value of FRAP assay respectively were 5.56%, 10.84 %, 12.50%. The flavonoid content respectively were 16.20 μg QAE/100% sample; 0.82 μg QAE/100% sample; 2.97 μg QAE/100% sample. The phenol content respectively were 4.88 μg GAE/100% sample; 0.19 μg GAE/100% sample; 0.28 μg GAE/100% sample. Telang tea contain highest flavonoid, phenol, have highest antioxidant compared with TeMon and lemon tea. TeMon tea show lower antioxidant activity compared with telang tea but TeMon have tasty and attractive color.