Characterization of antigen-specific B cells using nominal antigen-coated flow-beads

15Citations
Citations of this article
68Readers
Mendeley users who have this article in their library.

Abstract

In order to characterize the reactivity of B cells against nominal antigens, a method based on the coupling of antigens onto the surface of fluorescent core polystyrene beads was developed. We first demonstrate that murine B cells with a human MOG-specific BCR are able to interact with MOG-coated beads and do not recognize beads coated with human albumin or pp65. B cells purified from human healthy volunteer blood or immunized individuals were tested for their ability to interact with various nominal antigens, including viral, vaccine, self and alloantigens, chosen for their usefulness in studying a variety of pathological processes. A substantial amount of B cells binding self-antigen MOG-coated beads can be detected in normal blood. Furthermore, greater frequencies of B cell against anti-Tetanic Toxin or anti-EBNA1 were observed in primed individuals. This method can reveal increased frequencies of anti-HLA committed B cells in patients with circulating anti-HLA antibodies compared to unsensitized patients and normal individuals. Of interest, those specific CD19 cells were preferentially identified within CD27 -IgD+ (i-e naïve) subset. These observations suggest that a broad range of medical situations could benefit from a tool that allows the detection, the quantification and the characterization of antigen-specific blood B cells. © 2013 Degauque et al.

Cite

CITATION STYLE

APA

Degauque, N., Ngono, A. E., Akl, A., Lepetit, M., Crochette, R., Giral, M., … Soulillou, J. P. (2013). Characterization of antigen-specific B cells using nominal antigen-coated flow-beads. PLoS ONE, 8(12). https://doi.org/10.1371/journal.pone.0084273

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free