Co-52 resistance allele contributes to induce basal defense against Colletotrichum lindemuthianum race 71

Abstract

The role of the Co-52 and Co-42 alleles in the early activation of bean immunity against Colletotrichum lindemuthianum is poorly known. Thus, by using the anthracnose-susceptible Sutagao cultivar and the anthracnose-resistant bean G2333, this study aimed to evaluate the presence of the Co-42 and Co-52 alleles by SCAR markers, as well as their role in the respective defense responses, using histochemical tests and differential gene expression. The results showed that the Co-52 allele contributes to defense responses against anthracnose, since susceptible beans with the Co-42 allele and without the Co-52 allele decreased both the number of callose deposits and the accumulation of reactive oxygen species at the fungus attack sites. Additionally, for the Sutagao cultivar, there was a late response of defense-related genes such as PR-1, PR-3 and Pv-POD. The Co-52 allele contributes to induce defense mechanisms in the Phaseolus vulgaris and C. lindemuthianum interaction