Isotope discrimination of carbonyl sulfide (34S) and carbon dioxide (13C, 18O) during plant uptake in flow-through chamber experiments

Abstract

Carbonyl sulfide (COS) has been proposed as a proxy for gross primary production (GPP), as it is taken up by plants through a pathway comparable to that of CO2. COS diffuses into the leaf, where it undergoes an essentially one-way reaction in the mesophyll cells, irreversibly catalyzed by the enzyme carbonic anhydrase (CA), and is likely not respired by the leaf. In order to use COS as a proxy for GPP, the mechanisms of COS uptake and its coupling to photosynthesis need to be well understood. Characterizing the isotopic discrimination of COS during plant uptake could provide valuable information on the physiological COS uptake process and may help to constrain the COS budget. This study presents joint measurements of isotope discrimination during plant uptake for COS (CO34S) and CO2 (13CO2 and C18O16O). A C3 plant, sunflower (Helianthus annuus), and a C4 plant, papyrus (Cyperus papyrus), were enclosed in a flow-through plant chamber and exposed to varying light levels. The incoming and outgoing gas compositions were measured online, and discrete air samples were taken for isotope analysis. Simultaneously measuring fluxes and isotope discrimination of both COS and CO2 yielded a unique dataset that includes information on the plant's behavior and allowed for the estimation of stomatal- and mesophyll conductances. The average COS uptake fluxes were 73.3 ± 1.5 pmol m-2 s-1 for sunflower and 107.3 ± 1.5 pmol m-2 s-1 for papyrus (PAR > 0) and displayed virtually no trend with increasing PAR from 200 to 600 μmol m-2 s-1. The mean observed 34 "for COS was 3.4 ± 1.0 ‰ for sunflower and 2.6 ± 1.0 ‰ for papyrus. 34 "was stable across all light intensities, which could be explained by a sufficient stomatal opening and low variability in the ratio of mesophyll vs. ambient COS mole fraction, CmS/CaS. For both C3 and C4 plants, for CO2, a negative relationship was observed between the uptake flux and the isotopic discriminations 13 "and 18 ". The CO2 uptake and 13CO2 and C16O18O discriminations of sunflower have expected values for a C3 plant, while the low CO2 flux and high 13 "and 18 "values observed for papyrus were not in the typical C4 range, which was perhaps due to the relatively low light conditions during our experiments.