Sediminicola arcticus sp. nov., a psychrophilic bacterium isolated from deep-sea sediment, and emended description of the genus sediminicola

Abstract

A Gram-stain-negative, rod-shaped and non-motile strain, designated PAMC 27266 T , was isolated from deep-sea sediment of the Arctic Ocean. Analysis of the 16S rRNA gene sequence of strain PAMC 27266T showed closest affiliation with the genus Sediminicola. Phylogenetic analyses revealed that strain PAMC 27266T formed a robust clade with Sediminicola luteus CNI3T , with which it shared 98.9 % 16S rRNA gene sequence similarity. Genomic relatedness analyses based on the average nucleotide identity and genome-to-genome distance showed that strain PAMC 27266T is clearly distinguished from S. luteus. Cells of strain PAMC 27266T grew optimally at 15 6C and pH 6.5-7.5 in the presence of 3.5 % (w/v) sea salts. The major polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids and two unidentified lipids. The only respiratory quinone was menaquinone-6. The major cellular fatty acids (.10 %) were C16 : 1ω6c and/or C 16 : 1ω7c and C15 : 0. The genomic DNA G+C content was 37.9 mol%. Based on the phylogenetic, genomic, chemotaxonomic and phenotypic data presented, we propose strain PAMC 27266T (=KCCM 43038T=JCM 19894T) as the type strain of a novel species, with the name Sediminicola arcticus sp. nov.