Isolation and Identification of Fructo-Oligosaccharides in Roots of Asparagus (Asparagus Officinalis L.)

Abstract

Eight fructo-oligosaccharides were isolated from purified oligosaccharide fractions of the roots of Asparagus officinalis L. (Liliaceae). By examination of constituent sugars, gasliquid-chromatographic analysis of methyl derivatives, and investigation of partial acid hydrolyzates and products of β-fructofuranosidase action, they were confirmed to be 1F(1-β-fructofuranosyl)n sucrose [n=1 (1-kestose), 2 (nystose), and 3], 6G(1-β-fructofuranosyl) n sucrose [n=1 (neokestose), 2, and 3], 1F, 6G-di-β-fructofuranosyl sucrose, and a new pentasaccharide 1F(1-β-fructofuranosyl)2-6G-β-fructofuranosyl sucrose. © 1976, Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved.