A duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains

Abstract

Introduction: This study aimed to develop a duplex endpoint PCR assay for rapid detection and differentiation of Leptospira strains. Methods: Primers were designed to target the rrs (LG1/LG2) and ligB (LP1/LP2) genes to confirm the presence of the Leptospira genus and the pathogenic species, respectively. Results: The assay showed 100% specificity against 17 Leptospira strains with a limit of detection of 23.1pg/µl of leptospiral DNA and sensitivity of 103 leptospires/ml in both spiked urine and water. Conclusions: Our duplex endpoint PCR assay is suitable for rapid early detection of Leptospira with high sensitivity and specificity.