Inhibition of Herpes Simplex Virus Type 2 In Vitro by Durian ( Durio z ibethinus Murray) Seed Coat Crude Extracts

Abstract

The aim of this study was to determine Herpes simplex virus type 2 (HSV-2G) inhibition by durian seed coat crude extracts. Dried native durian seed coat from Lab-Lae District, Uttaradit Province was extracted with solvents; distilled water and ethanol. Subsequently, two durian seed coat crude extracts were investigated their cytotoxicity on Vero cells and determined HSV-2G inhibition by plaque reduction assay. The results displayed that 50%cytotoxicity dose (CD 50 ) of aqueous and ethanolic extracts of durian seed coat were 224.40 and 112.47 μg/ml, respectively. Moreover, the aqueous and ethanolic extracts of durian seed coat at a concentration of 100 μg/ml could inhibit HSV-2G by 36.34 ± 1.47 and 61.56 ± 5.30%, respectively. This study displayed the one of advantages of native seed durian in Uttaradit.