A simple, rapid and very efficient protocol for DNA isolation from mosquito species

Abstract

We represent a simplified and rapid version of DNA extraction protocol from different mosquito species which is suitable for polymerase chain reaction(PCR) and other molecular biology works.The protocol involves three steps like lysis, phenol : chloroform (1:1) extraction and two fold isopropanol precipitation at -20 degree celcius using 1X STE buffer (50mM NaCl, 50mM Tris- Hcl,100mM EDTA,PH 8.0).The proposed extraction protocol has an advantage of DNA extraction from mosquitoes using 1X STE buffer at 37ºcelcius which prevent DNA degradation at higher temperature and kept DNA stability in long term storage . The protocol proved by three different mosquito species and removing for potential contamination showed that the protocol yields good quantity and quality DNA, typically better than commercial kits .The protocol was evaluated by polymerase chain reaction (PCR). The protocol of DNA extraction is a time saving as well as economies with available laboratory chemicals, consumables and basic equipments. The protocol is adoptable for laboratories in external and internal funded research projects with large numbers of mosquito samples in a small period .The protocol minimize the time for other DNA extraction protocols in two to three days and provides a workflow for mapping of malaria vectors, their vectorial transmission in the area of translational research.