An in vitro system of indole-3-acetic acid formation from tryptophan in maize (Zea mays) coleoptile extracts

Abstract

The formation of a product from tryptophan that had the same retention time as that of authentic indole-3-acetic acid (IAA) on high performance liquid chromatography was detected in crude extracts of maize (Zea mays) coleoptiles. The product was identified as IAA by mass spectrometry. The IAA-forming activity was co-purified with an indole-3-acetaldehyde (IAAId) oxidase activity by chromatography on Hydrophobic and gel filtration (GPC-100) columns. During purification, the IAA-forming activity, rather than that of IAAId oxidase, decreased; but when hemoprotein obtained from the same tissue was added, activity recovered to the same level as that of IAAId oxidase. The promotive activity of the hemoprotein was confirmed by the result that the activity coincided with amounts of the hemoprotein after GPC-100 column chromatography. The hemoprotein was characterized and identified as a cytosolic ascorbate peroxidase (T. Koshiba [1993] Plant Cell Physiol [in press]). The reaction of the IAA-forming activity was apparently one step from tryptophan. The activity was inhibited by 2-mercaptoethanol. The optimum temperature for the IAA-forming system as well as for the IAAId oxidase was 50 to 60°C, and the activity at 30°C was one-third to one-half of that at 60°C. The system did not discriminate the L- and D-enantiomers of tryptophan.