α–AMYRIN AND β-SITOSTEROL FROM BARK EXTRACT OF RHIZOPHORA MUCRONATA LAMK. AND THEIR CYTOTOXIC ACTIVITIES AGAINST HELA CELL LINE

Abstract

The aims of this study are to isolate and identify active compds. of mangrove plants Rhizophora mucronata Lamk bark ext. obtained from the mangrove forest of Kendari Bay, Southeast Sulawesi Province Indonesia and study their cytotoxic activities against cervical cancer (HeLa) cell line. The isolation and purifn. of the compds. were carried out under several chromatog. methods, including thin layer chromatog., vacuum liq. chromatog. and radial chromatog. with silica gel as adsorbent and various solvents mixt. as an eluent. Elucidation of the structure of the isolated compds. was done based on FTIR, 1H-NMR, 13C-NMR (1D and 2D) spectroscopic data and confirmed with an existed data from literatures. From this research, it was obtained two pure compds., α-amyrin (1) and β-sitosterol (2). The isolation of this α-amyrin was firstly reported so far from the bark of this plant. Cytotoxic activities, marks by an IC50 value, of both α-amyrin and β-sitosterol are 804.762 ± 0.22 μg/mL and 353.871 ± 0.53 μg/mL resp. and shows that cytotoxic of β-sitosterol is stronger than α-amyrin. [on SciFinder(R)]