Ubiquitin-interacting motifs confer full catalytic activity, but not ubiquitin chain substrate specificity, to deubiquitinating enzyme USP37

Abstract

Background: The role of ubiquitin-interacting motifs (UIMs) in the deubiquitinating enzyme USP37 is unknown. Results: Inactivation of the UIMs in USP37 resulted in lower isopeptidase activity toward ubiquitin chains. Conclusion: The UIMs in USP37 are required for the full catalytic activity of the enzyme. Significance: This study reveals a novel mechanism to increase the catalytic activity of deubiquitinating enzymes. © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.