Micropropagação e germinação de sementes in vitro de atroveran

Abstract

Atroveran (Ocimum selloi Benth. - Lamiaceae) is medicinal plant used for digestive disturbs and inflammation. The objectives of this study were to establish nodal segments, seed germination and in vitro plantlet growth on sucrose and different basal salt concentrations, as well as plantlet acclimatization in different substrates. For establishment and growth, three different basal salt formulations (MS, 1/2MS and 1/4MS) and two concentration of sucrose (15g/L, 30g/L and control) were used. For seed germination, MS, 1/2MS, 1/4MS and 0MS (control) and two sucrose (15g/L, 30g/L and control) were used. For acclimatization, the substrates sand, Plantmax ®, soil and soil +cattle manure (2:1) were used. The results indicated 15g/L of sucrose to establish the explant and nodal segment growth. The 1/2MS basal salt formulation was better to establish the explant and 1/4MS to nodal segment growth of O. selloi. The results indicated higher seed germination percentage and germination speed index on medium withoutsucrose. For growth up to 30 days better results were observed on 1/4MS without sucrose and for plantlet to maintain growth of O. selloi in vitro the better results indicated 1/4MS supplemented with 15g/L of sucrose. Plantlet acclimatization of O. selloi in the substratum Plantmax ® gave better growth.