Amycolatopsis sp. strain MN235945 Mediated Biosynthesis of Silver Nanoparticles: Characterization, Antimicrobial and Anticancer Activity against HeLa and MCF-7 Cell Lines

Abstract

Swamy et al.: Anticancer activity of Amycolatopsis sp. mediated Silver Nanoparticles The current study investigated about the biogenic synthesis and evaluation of biological activities of Amycolatopsis sp. strain MN235945 fabricated silver nanoparticles and their characterization by ultraviolet-visible spectroscopy, fourier transform infrared spectroscopy, high resolution-transmission electron microscopy, energy dispersive X-ray spectroscopy, X-ray diffractometry and zeta potential analysis. The most potent isolate Amycolatopsis sp. strain MN235945 was identified by morphological and genotypic characterization and biological activity of silver nanoparticles was studied via antimicrobial and anticancer activity assays. The synthesized silver nanoparticles with spherical shape and polydispersed nature showed ultraviolet visible spectroscopy, absorption maximum at 417 nm with the smallest size of 19.40 nm. The presence of various biological functional groups and different elemental composition of silver nanoparticles were determined along with zeta potential value of-34.8 mV; whereas, the X-ray diffractometry analysis revealed the specific Bragg's diffraction peaks for metallic silver. The silver nanoparticles with increased antimicrobial activity significantly inhibited cervical adenocarcinoma HeLa and human breast cancer MCF-7 tumour cells with IC 50 values of 81.12 and 235.45 µg/ml respectively. These encouraging results obtained via low-cost green synthesis of silver nanoparticles from marine Amycolatopsis sp. strain MN235945 provide helpful information for further research and designing an anticancer agent in coming years using Amycolatopsis sp. strain MN235945 mediated silver nanoparticles with minimal side effects. Nanotechnology is an essential field in recent days, where it is dealing with many research like synthesis, characterization and different shapes, sizes (1-100 nm) of the nanoparticles. Several studies regarding the synthesis of biological nanoparticles using various microorganisms like fungi, Actinomycetes, bacteria, viruses and different plant parts are investigated. It shows promising potential for controlling different pathogens. With multiple applications, nanoparticle research plays a vital role in the fields such as cancer study [1] , larvicidal and insecticidal activity [2] and plant disease control in agricultural sciences [3]. Biological activities like antibacterial, antifungal, anticancer [4] , anti-inflammatory, antiviral, cardioprotection [5] , wound healing [6] , and biosurfactants [7] helped nanotechnology to gain enormous popularity within a short time. Silver nanoparticles (AgNPs) have been used in various applications like cosmetics, solar cells, catalysts, ceramics, gas sensors, anti-bacterial properties, energy sector plastics, environmental protection [8] , drug delivery and bioimaging, textiles, probes, commercial products such as pastes, food packaging, soaps, optical and electrical properties, toxicity against cancer cells [9,10]. There are varieties of methods like physical, chemical and biological approaches to synthesize AgNPs and biological synthesis of AgNPs is more opted [11,12]. The AgNPs synthesis using various microorganisms are well documented in Streptomyces rochei MHM13 [13] , Aspergillus tamarii [14] , Penicillium atramentosum KM [15] , Acinetobacter baumannii [16] , Gordonia amicalis HS11 [17] , and Escherichia coli [18] , Lactobacillus plantarum TA4 [19] and from Enhalus acoroides [20] .