Isolation, Production and Optimization of Siderophores (Iron Chilators) from Pseudomonas fluorescence NCIM 5096 and Pseudomonas from Soil Rhizosphere and Marine Water

Abstract

Two Pseudomonas spp. are isolated from different natural sources i.e. soil rhizosphere and marine water on selective media like cetrimide agar base and King’s B medium. Pseudomonas fluorescence NCIM 5096 and other two isolated of Pseudomonas produced siderophore with the yield 71\%, 72.33\%, 33\% of hydroxamate type, in iron free succinate medium (SM). Nutrient medium inhibited the growth of siderophore while the succinate medium gave the maximum production of siderophore. pH has important role in production of siderophore i.e. at neutral pH 7; maximum siderophore yield while at acidic pH production was found ceasing. Succinate medium supplemented with Pb showed enhancement while other supplemented with Co showed inhibition of production of siderophore. Isolated siderophore showed the antagonists effects against human pathogenic Pseudomonas aeruginosa and on phytopathogenic fungi.