In vitro antiproliferative effect of aqueous extract of solanum macranthum fruits on mda-mb-231 tripple negative breast cancer cell line

Abstract

The current investigation was focused on the assessment of antiproliferative effects of the Solanum macranthum aqueous fruits extract against the human breast cancer cell line MDA-MB-231 in vitro. Phytochemical analysis of the S. macranthum aqueous fruits extract was performed and the phytoconstituents found positive for alkaloids, phenols, and saponins. Quantitative analysis of the total alkaloid and phenol was determined using gravimetric analysis and folins-phenols reagent method, respectively. The total alkaloid present was estimated to be 7.58% and total phenol of 158.774 mg/Gallic Acid Equivalent. The cytotoxicity of S. macranthum aqueous fruits extract was screened using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay and camptothecin was used as standard reference drug. At the concentration of 500 µg/ml, the aqueous extract potentially inhibited the growth of the MDA-MB-231 cell line in vitro reaching 19%, and the IC50 value was calculated to be 525.59 μg/ml. Thus, indicating that aqueous extract of the S. macranthum fruits possess potential anticancer property.