Anticancer Activity of Tabernaemontana coronaria against Carcinogen Induced Clear Cell Renal Cell Carcinoma

Abstract

Medicinal plants have been used for treatment of various ailments since time immemorial. Tabernaemontana coronaria is a flowering plant belonging to Apocynaceae family. Considering the very good antioxidant properties of Tabernaemontana coronaria , the anticancer property of the ethanolic extract was evaluated against DEN and Fe-NTA induced clear cell renal cell carcinoma. A group of male rats were induced with single intraperitoneal injection of DEN (200 mg/kg bw) and followed by Fe-NTA (9 mg Fe/kg bw) twice a week for 24 weeks. Another set of two groups were induced with carcinogens and treated with plant extracts (200 and 400 mg/kg bw), respectively. Further two sets of rats were treated with only the plant extracts. The therapeutic efficacy of the ethanolic extract of Tabernaemontana coronaria was observed in terms of normalization of altered renal oxidative stress parameters like LPO and enzymic and nonenzymic antioxidants in kidney of rats. These results suggested that the plant extract could act against DEN and Fe-NTA induced clear cell renal cell carcinoma in male rats by a mechanism related to its antioxidant properties which was confirmed by histopathological studies.