Influence of herbicide 2,4-D dimethylamine 865SL on photosynthetic mechanism of algae species Chlorella Kessleri immobilized in a biochip

Abstract

Algae are very important to the life of human as a bio-indicator of water pollution. The observation of algae vitality can give information of the environment changes of the algae, which are shown by the changes of parameter in its environment. This study is aimed to observe the influence of toxicity of herbicide 2,4-D dimethylamine 865 SL on the vitality of algae species Chlorella Kessleri in producing dissolved oxygen as a result of photosynthetic mechanism. A 50μL herbicide 2,4-D dimethylamine 865 SL with a concentration of 10 % was added into immobilized algae in the Biochip-C from cellasys GmbH and stimulated with artificial light of 400-700nm for photosynthetic process. The effect of herbicide 2,4-D dimethylamine 865 SL was observed and shows the increase of the basal potential after 1500 s, which indicates the dissolved oxygen reduction in the environment of the algae. This effect is reversible and a restoration of the photosynthetic activity take place after the substance removal. The use of this toxin is systemic, which slowly kill the living cells.