MP192EVALUATION OF AEROBIC EXERCISE ON THE RECOVERY PHASE OF ACUTE KIDNEY INJURY INDUCED BY GENTAMICIN IN RATS

Abstract

Introduction and Aims: Acute kidney injury is a serious public health problem, especially in intensive care units, where patients may require dialysis support, resulting in 50% mortality. The objective of this study was to evaluate the effects of aerobic exercises on the recovery phase of acute kidney injury induced by gentamicin in rats. Methods: Male adult Wistar rats were allocated into 4 groups: W10+R30, G10+R30, W10+EX30 and G10+EX30; in which W10 received water (gentamicin vehicle) and G10 received gentamicin for 10 days; R30 remained resting and EX30 made exercise for 30 days. Training was performed on treadmill. The blood and 24hr urine were collected pre and post training for creatinine, urea, proteinuria, nitric oxide, thiobarbituric acid reactive substances, reactive oxygen species and antioxidant analysis. Post training, kidneys were removed for oxidative stress, antioxidant and histological analysis. Results: After 10 days of gentamicin treatment, renal function was decreased, but it was recovered after R30 or EX30; nitric oxide synthesis was increased in the plasma and urine, and decreased in kidney of G10+R30 and G10-EX30 groups. The plasma, urinary and renal thiobarbituric acid reactive substances were reduced in G10+EX30 vs G10+R30; catalase, glutathione and superoxide dismutase were increased and histological analysis showed no difference between these groups. Conclusions: Our study suggests that the use of a non-pharmacological tool such as aerobic training appears to be safe in animals with G induced AKI, since it did not worsen the renal function, contributed to redox balance and restored the NO levels. Many studies have demonstrated beneficial effects of exercise in chronic kidney disease but, to our knowledge, it is the first time that it was showed the use of moderate exercise in AKI, reducing OS and increasing the antioxidant profile. We believe that these findings could contribute to prevent a further deterioration of renal function that could occur later and/or to protect the kidney against new insults. Ultimately, more studies are needed to identify antioxidant mechanisms that may be associated with the exercise and could provide new therapeutic targets in this pathological condition.