Histology of hematoxylin–eosin and immunohistochemical diabetes rat pancreas after giving combination of moringa leaves (Moringa oleifera) and clove flower (syzygium aromaticum) extracts

Abstract

AIM: The purpose of this study was to determine the regeneration activity of pancreatic beta cells by a combination of the ethanol extracts of moringa leaves and clove flower. METHODS: This is experimental laboratory research using posttest only group design. The subject of this research is 40 white male rats of Wistar strain (Rattus norvegicus) and was conditioned diabetes mellitus by streptozotocin-nicotinamide induction. The rats randomly divided into 8 groups, and each group consisted of 5 rats. Combination dose of moringa leaves and clove flower extracts used are 150:40 mg/kg body weight of mice, 100:80 mg/kg body weight of mice, and 50:120 mg/kg body weight of mice. The rats were sacrificed and the pancreas taken to be made preparations histopathology, staining, and observation tissues with hematoxylin–eosin (HE) and immunohistochemical (IHC) methods. RESULTS: The results showed that the staining and observation tissues of heart with HE method acquired the positive control group, the single test group and the combination group was showing the islet on the Langerhans Island of the pancreas is bigger than in the negative control group. CONCLUSION: The morphology of the island of Langerhans increasingly large indicates increasing the regeneration of the cells of the islet beta pancreas, whereas the method of IHC acquired the negative control group seen lack of display intensity of positive reaction antigen (Ag) and antibody (Ab) beta cells with are marked with brown cells. In the positive control group, the single test group and the combination group, seen a positive reaction of Ag and Ab insulin-producing beta-cells characterized by brown cells and insulin expression dominating pancreatic island of Langerhans.