MicroRNA expression profiles in C6 glioblastoma cell line treated with antipsychotics

Abstract

In the present investigation antipsychotic drug-induced microRNA modulations in glial cells were analysed through microRNA expression in antipsychotic drugs-treated C6 cells using a microRNA microarray. Seventeen miRNAs were upregulated by haloperidol and 10 miRNAs were upregulated by risperidone in C6 cells. Thirty-six microRNAs were downregulated by clozapine in C6 cells. Six microRNAs (rno-let-7a-5p, rno-miR-221-3p, rno-miR-152-3p, rno-miR-345-5p, rno-miR-3473 and rno-miR-551b-5p) were selected for validation by reverse transcription-quantitative polymerase chain reaction, the results of which showed almost the same expression trends as the microarray results. MicroRNA and protein expression of potential targets for some validated microRNAs was examined using reverse transcription-quantitative polymerase chain reaction, western blot analysis and immunofluorescence staining. Reduction of microRNA (rno-let-7a-5p, rno-miR-152-3p and rno-miR-221-3p) caused by clozapine treatment could further induce expressions of predicted target genes/proteins (atg12, cdkn1b, c-myc and K-ras) in C6 cells. MicroRNAs regulation induced by haloperidol or risperidone were not closely related to predicted target genes/proteins expressions. It was concluded that the regulatory trends between antipsychotic drug-induced microRNA and the mRNA/protein expression of potential targets of the examined microRNAs were not quite the same. Clozapine, but not haloperidol or risperidone, could modulate microRNA expression to further regulate protein expression in C6 cells.