Antibacterial activity of pasteurized milk supplemented with binahong leaf extract (Anredera cordifolia (Ten) Steenis) and sukrose toward Escherichia coli and Staphylococcus aureus

Abstract

The functional quality of pasteurized milk need to be improved by giving natural ingredients such as the addition of binahong leaf extract and sucrose. The purpose of this study was to determine the concentration of binahong leaf extract and the appropriate sucrose on the antibacterial activity of Escherichia coli and Staphylococcus aureus. This study uses a completely randomized design (CRD) factorial pattern with 2 (two) factors. The first factor (A) is the concentration of Binahong leaf extract (0%, 4%, 5%, 6%) and the second factor (B) is the concentration of sucrose (0%, 5%, 7%, 9%) of each treatment. repeated 3 times. Antibacterial activity of binahong leaf extract significantly affected the inhibition of Escherichia coli and Staphylococcus aureus. Inhibition of pasteurized milk against Escherichia coli and Staphylococcus aureus was not affected by sucrose antibacterial activity and the intrusion between the two treatments (binahong leaf extract and sucrose). Binahong leaf extract concentration of 5-6% can increase inhibition of Escherichia coli and Staphylococcus aureus.