EXTRACTION OF 4H-PYRAN-4-ONE, 2,3- DIHYDRO -6-METHYL-, AN ALTERNATIVE ANTIFUNGAL AGENT, FROM SCHIZOPHYLLUM COMMUNE: OPTIMIZATION AND KINETIC STUDY

Abstract

4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP) was believed as a promising alternative in term antifungal activity towards fungal attack in rubberwood. Solid-liquid extraction is performed from basidiomycetes fungus Schizophyllum commune in methanol-water solvent, in order to obtain valuable antifungal agent. Statistical optimization was employed to optimize the extraction condition for maximal total flavonoid content (TFC) and DDMP productivity. The optimum conditions were 70.75% (v/v) methanol, 29 °C, and 145 rpm. The optimization studies were verified and the experimental data fitted well to the selected models with error percentage less than 1%. The extraction kinetics was theninvestigated using Parabolic diffusion model, Power law model, Peleg’s model, and Elovich’smodel. All empirical models gave a good fit to the experimental data (R2 > 0.9), in which the Power law model having the highest R2 and lowest RMSD values.