Producción de Ácidos Grasos Poliinsaturados a partir de Biomasa Microalgal en un Cultivo Heterotrófico

Abstract

In this research, we obtained Polyunsaturated Fatty Acids (PUFAs) from Microalgal Biomass in heterotrophic culture. In order to obtain these PUFA’s we used Chlorella sp. and Scenedesmus sp. strains with heterotrophic conditions. Subsequently, we selected the strain with higher productivity and determined the growth kinetics and biomass, glucose, phosphorus and nitrogen yields and the lipids extracted analyzed by gas chromatography. The heterotrophic culture was established in 1L Continuos Stirred-Tank Reactor (CSTR) under specific conditions; 28°C, 1vvm, pH 6,8 and a relation C/N 12:1. Then, we performed sub-cultivation in a “Bioreactor BioFlo115” with 10L volume and made productivity analysis of lipids. Lipids profile allowed us to determine that higher Fatty Acid present in Chlorella sp. is oleic acid (C 18:1) and represented a percentage of 28,75 of total fatty acids. Also we noted the accumulation the other fatty acids like; palmitoleic acid (C 16:1) 19.75%, arachidonic acid (C 20:0) 19.37%, linoleic acid (C 18:2) 11.86%, palmitic acid (C 16:0) 7.24%, linoleic acid (ɤ-C 18:3), 2.61%, erucic acid (C 22:1) 4.61%, stearic acid (C 18:0) 2.4%.