UPLC-Q-TOF/MS analysis of chemical constituents of methanolic extract from cassava tender stems

Abstract

In the processing and utilisation of cassava, stems and leaves usually become waste. The tender stems of cassava were analysed by electrospray ionisation high-performance liquid chromatography-tandem mass spectrometry. Using UPLC BEH C18 column (50 × 2.1 mm; i.d.: 1.7 μm), the mobile phases were H2O (0.1% FA) and CH3CN (0.1% FA), the column temperature was 30°C, and the flow rate was 0.30 mL/min. Mass spectrometry was performed by the Waters SYNAPT G2 HDMS system. This gave excellent reproducibility and avoided the interference of some similar substances. A total of 33 compounds were identified in Bzhe samples of cassava tender stems by this method, mainly cyanosides, catechins, flavonoids, glycosides, glycosides, phospholipids, and other components. The present work provided reference value for the development and utilisation of cassava tender stems.