Development of a nano-particle-assisted PCR assay for detection of duck tembusu virus

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Abstract

Duck tembusu virus (DTMUV) has caused significant economic losses to the poultry industry in China since the spring of 2010. In this study, a nano-PCR assay targeting E gene of DTMUV was developed and their sensitivities and specificities were investigated. Under the optimized conditions of nano-PCR assay for detection of DTMUV, the nano-PCR assay was 10-fold more sensitive than a conventional PCR assay. The lower detection limit of the nano-PCR assay was 18 × 102 copies μl-1 of DTMUV RNA, as no cross-reaction was observed with other viruses. This is the first report to demonstrate the application of a nano-PCR assay for the detection of DTMUV. The sensitive, and specific nano-PCR assay developed in this study can be applied widely in clinical diagnosis and field surveillance of DTMUV-infection.

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Wanzhe, Y., Jianuan, L., Peng, L., Jiguo, S., Ligong, C., & Juxiang, L. (2016). Development of a nano-particle-assisted PCR assay for detection of duck tembusu virus. Letters in Applied Microbiology, 62(1), 63–67. https://doi.org/10.1111/lam.12509

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