A proteome map of the pituitary melanotrope cell activated by black-background adaptation of Xenopus laevis

Abstract

Upon transfer of Xenopus laevis from a white to a black background, the melanotrope cells in the pituitary pars intermedia secrete α-melanocyte- stimulating hormone, which stimulates dispersion of melanin pigment in skin melanophores. This adaptive behavior is under the control of neurotransmitters and neuropeptides of hypothalamic origin. The α-melanocyte-stimulating hormone-producing cells and their hypothalamic control system provide an interesting model to study proteins required for biosynthetic and secretory processes involved in peptide hormone production and for brain-pituitary signaling. We present a 2-D PAGE-based proteome map of melanotrope cells from black-adapted animals, identifying 204 different proteins by MS analysis. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.