Excretion of cannabinoids in urine after ingestion of cannabis seed oil

Abstract

Gas chromatographic-mass spectrometric (GC-MS) quantitation of 25 cannabis sed oils determined Δ9-tetrahydrocannabinol (THC) concentrations from 3 to 1500 μg/g oil. In a pilot study, the morning urine of six volunteers who had ingested 11 or 22 g of the oil, which contained the highest THC content (1500 μg/g), was collected for six days. The urine samples were screened by immunoassay, and the content of 11-nor-9-carboxy- Δ9-THC (THCCOOH) was determined by GC-MS. Urine samples were found cannabis positive for up to six days with THCCOOH-equivalent concentrations up to 243 ng/mL by the Abuscreen OnLine immunoassay and THCCOOH contents from 5 to 431 ng/mL by the GC-MS method. All subjects reported THC-specific psychotropic effects.