Egg white lysozyme promoted collagen secreting of dermal fibroblasts in mice

Abstract

The purpose of this study was carried out the effects of egg white lysozyme treatment on collagen secreting in mice skin. As one of the most indispensable constituting components of skin, collagen is mainly proved to be responsible for its tensile strength. By far, results have shown that lysozyme, an antibacterial enzyme, plays some positive role in skin burn and yet its function has not been explained in detail in regards to skin condition. In this study, the role of egg white lysozyme in collagen biosynthesis was examined by both dermal fibroblasts in vivo and in vitro. RT-PCR was used to trace the expression levels of collagen in cultured cells and HE and VG staining assessed the collagen content in dermis in vivo. According to present study, egg white lysozyme is discovered to promote the secretion of collagen in fibroblasts treated with a dosage of 10 mg mL-1 around 12 to 48 h. Though, it was found that both he expression levels of collal and col3al were increased in present experiment, yet the ratio between the secreted type-I collagen and type-cocollagen remained the same in fibroblasts treated with egg white lysozyme. In addition, higher skin moisture content was observed in the mice group treated with egg white lysozyme in vivo according to the report that egg white lysozyme might enhance the hyaluronan synthase1 (Has1) expression. Moreover, in the histological tests the collagen content also increased after egg white lysozyme treatment in both young and old groups with collagen fibril arranged more orderly and compactly than the control group. Egg white lysozyme promoted collagen biosynthesis and extracellular matrix proteins by fibroblasts in the dermis. © 2011 Academic Journals Inc.