Optimization of estrogenic activities of kebar grassextract (Biophytum petersianum) on white female mice (mus musculus)

Abstract

Menopause occurs caused bythe decline of estrogen hormone due to the depletion of the ovarian egg. As estrogen levels decrease, the risk of osteoporosis, bone fractures, heart disease and even cancer increase. The kebar grass (Biophytum petersianum), which has long been used as atraditional fertility medicine by Papuans, is suspected to have anestrogenic activity. The purpose of this study was to determine the estrogenic activity of kebar grass extract on female mice (Musmusculus). Five experimental female mice groups were used in this study. Each group consisted of 5 mice.The negative control group was administered with aquadest; the positive control was administered withesthero conjugated estrogen 0.625 mg; the first group (P1) was administered with 3%kebargrass extract; the (P2) group was administered with 5% kebar grass extract and the (P3) was administered with 10% kebar grass extract. The experiment was carried out for 7days. The results of the study showed that the kebar grass extract has an estrogenic effects on female mice include to shorten the estrous cycle, prolong the estrous phase, improving the vascularity of ovary and uterus, and increase the weight of ovary and uterus. The highest estrogenic activity was reached when the mice was administered with 10% kebar grass extract.