Histologic response of aortic Rattus norvegicus male strain wistar hyperlipidemia after giving kersen fruits juice and extract lakum leaves

Abstract

People lifestyle who tend to consume high fat diet is one of the risk factors causing cardiovascular diseases such as atherosclerosis and coronary heart disease. The aims of this study were to analyze the effect of kersenfruits juice and extract lakum leaves on lumen width and thickness of aortic wall and to find out the histological features of male Rattusnorvegicus white rats due to hiperlipemia. The study used a Completely Randomized Design (CRD) consisting of 5 treatments with 4 replications, i.e. the control group (P0), the standard feeding group (P1), the hyperlipid feed group+0.2 mL/200gBB kersen juice (P2), the hyperlipid feed group+40mg / 200gBB extract lakum leaves (P3) and hyperlipid feed group +0.18mg / 200gBB simvastatin (P4). Hyperlipid feeds were given for 28 days. Data were analyzed by ANOVA followed by Duncan Test at a confidence level of 95, while histological features were observed directly using photomicrograph with 400x magnification and were seen aortic lumen narrowing, foam cells and intracellular lipid accumulation in smooth muscle. The results showed that the addition of kersenfruits juice and extract lakum leaves gave a significant effect on lumen width and thickness of the aortic wall (p <0.05). Histological features show the presence of foam cells and accumulation of smooth muscle lipids in the control and group treatments with hyperlipemia feeding. The conclusion of this study is that the addition of kersen juice fruits and extract lakum leaves can reduce atherosclerotic lesions by eliminating foam cells and proliferating smooth muscle cells so the size of the aortic lumen width returns to normal.