PATHOGENESIS-RELATED PROTEIN INDUCTION IN Streptomyces GanoSA1-TREATED OIL PALM SEEDLINGS DURING EARLY INFECTION BY Ganoderma boninense

Abstract

Basal stem rot (BSR) disease in oil palm is caused by a fungal pathogen identified as Ganoderma boninense. In our previous study, Streptomyces nigrogriseolus GanoSA1 showed strong antagonistic activity against G. boninense PER71 in laboratory, nurseries, and field studies. This study aimed to identify the role of Streptomyces GanoSA1 in inducing pathogenesis-related proteins in oil palm seedlings during the early infection by the pathogen. Three-month-old commercial oil palm seedlings (D×P) were inoculated with Streptomyces GanoSA1 and artificially infected with G. boninense PER71 through the rubberwood block sitting technique. The seedlings were harvested at 0, 3, 7, 14, 28 and 60 days after inoculation (DAI) with G. boninense PER71. The seedlings were tested for the production of β-glucanase, peroxidase (POX), polyphenol oxidase (PPO), and phenylalanine lyase (PAL). The Streptomyces GanoSA1 significantly induced the production of β-glucanase (3.04-fold), POX (1.68-fold), PPO (1.88-fold), and PAL (1.127-fold) in oil palm roots compared to control seedlings. The biological control activity by Streptomyces GanoSA1 might be contributed by the induction of pathogenesis-related proteins.