A SIMPLE HPLC METHOD FOR THE DETERMINATION OF OMEPRAZOLE IN VITRO

Abstract

Omeprazole is used for the treatment of stomach and gastroesophageal reflux disease. Omperazole is administered in the form of oral dose as independent drug or in combination with other antibiotics. Present study was carried out to develop and validate a simple HPLC methtod of determination of omeprazole in vitro. Mobile phase empolyed was acetonitrile: phosphate buffer (65:35), pH 6.8 and C 18 column was used. UV detector was used using 300nm eluted with the mobile phase at a flow rate of 1.0 mL/min. percentage recovery was 99.92%±0.4%. This method was used for the routine analysis of omeprazole in raw material & finished product.