Histopatology of Cyprinus carpio tissue infected by Aeromonas hydrophila in profilactical therapy Pothos tener Wall

Abstract

Photos tener Wall is a semi-aquatic plant that lives in the Bantimurung waterfall, South Sulawesi. The aims of study to determine the effectiveness of P. tener Wall in prophylactic therapy against Aeromonas hydrophila infection in Cyprinus carpio. The treatment procedures were: a) Soaking the live P. tener Wall in aquarium at a dose of 15 gr, 30 gr, 60 gr wet weight, b) Provision of P. tener Wall through feed at a dose of 1%, 2% and 4%, c) mixed treatment of 30 gr P. tener Wall live and 4% P. tener Wall in feed. Positive control (d) C. carpio given oxytetracyline antibiotics and negative control (e) C. carpio without P. tener Wall treatment and without antibiotics. Histological observations of liver, kidney and lymph were carried out: a) Before prophylactic therapy (as a normal condition), b) 30th day of prophylactic therapy c) 7th day after A. hydrophila infection. The results of histological observations showed that C, carpio in normal condition. Treatment with oxytetracyline antibiotics showed a lot of tissue damage, fatty degeneration, haemorrhage, and no necrosis. Negative controls included more necrosis, fatty degeneration, tissue damage, haemorrhage, hyperplasia. The P. tener wall treatment had little of tissue damage compared to positive and negative controls. Conclusion Prophylactic therapy with P. tener Wall can emphasize and reduce tissue damage caused by A. hydrophila attack rather than antibiotic treatment.