Validasi Metode Matrix Solid Phase Dispersion (MSPD) Spektrofotometri UV untuk Analisis Residu Tetrasiklin dalam Daging Ayam Pedaging

Abstract

Method Validation Matrix Solid Phase Dispersion (MSPD)-Spektrofotometri UV for The Analysis of Residual Tetracycline in Meat Broilers. Tetracyline are antibiotics that have been widely used by poultryman to prevent diseases in dairy broiler chicken. Overuse of these antibiotics may lead to the presence of its residues in animal products including meat. Clinical implication that may occur such as allergy, intoxication, and spesific of antibiotic resistance. Tetracyclines residue in broiler chicken meat can be analyzed with ultraviolet spectrophotometer using standard addition technique. The meat was extracted with Matrix Solid Phase Dispersion (MSPD) method. Recovery was 84.40% according to standard addition method with acceptable limit 80-110%. Relative standar deviation were 1.36%. The limit of detection were 5.16 µg/ml and limit of quantitation were 5.54 µg/ml. The linearity test presented a correlation coefficient were 0.9997. The analysis of broiler chicken meat from three department store in Bandar Lampung city showed the amount of tetracyclines residue were 42.40 mg/kg; 61.05 mg/kg; and 44.47 mg/kg. These value has exceeded the maximum limit of tetracycline residues in meat according to SNI 01-6066-2000, ie 0.1 mg/kg.