Enantioanalysis of Leucine and Arginine: A Key Factor in Lung Cancer Metabolomics

Abstract

Metabolomics has become a key factor for identifying new biomarkers able to be used for early diagnosis of cancer. A zinc oxide- α -cyclodextrin nanocarbon nanocomposite 3D stochastic microsensor ( α -CD/ZnO/nanoC) was designed and integrated into an intelligent 3D stochastic enantioselective platform for the enantioanalysis of arginine and leucine in whole blood samples. The α -CD/ZnO/nanoC pastes were morphologicaly characterized by scanning electron microscopy, and electrochemically characterized by cyclic voltammetry and electrochemical impedance spectroscopy. L-Leucine was determined on a working concentration range between 1 × 10 −11 –1 × 10 −8 mol l −1 with a limit of detection of 3 × 10 −12 mol l −1 ; D-leucine was determined on a working concentration range between 1 × 10 −16 –1 × 10 −11 mol l −1 with a limit of detection of 3 × 10 −17 mol l −1 ; L-arginine was determined on a working concentration range between 1 × 10 −12 –1 × 10 −9 mol l −1 with a limit of detection of 3 × 10 −13 mol l −1 ; D-arginine was determined on a working concentration range between 1 × 10 −14 –1 × 10 −11 mol l −1 with a limit of detection of 3 × 10 −15 mol l −1 . High sensitivities, selectivities, and enantioselectivities were recorded when the platform was used for the enantioanalysis of arginine and leucine. High recoveries were recorded at the enantioanalysis of arginine/leucine, with low relative standard deviations.