Fast and efficient RP-HPLC method for simultaneous determination of water-soluble vitamins in some nutraceutical supplements

Abstract

THE determination purpose of of this water-soluble study is the vitamins development (B1, B2, B of 3, analytical B6, B9, B12 method and vitamin for simultaneous C) in dietary supplement syrup, multivitamins and mineral dietary supplement syrup with iron, dietary supplement capsules and balanced supplemental nutrition for children and adult gum by using gradient RP-HPLC methods with PDA detector andcalculating the uncertainty for each matrix of analytical results based on the information from the validation process. The separation of water-soluble vitamins was performed on waters Spherisorb ODS2 (250 mm×4.6mm, 5μm) column. The wavelength was 272 nm at room temperature and the detection limits ranged from 2.4 to 8.3 ng/µl. The average recovery was 98%-102 % and correlation coefficient (r) was 0.9997 to 1.