Increasing the carbon flux toward synthesis of short-chain-length-medium- chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the β-oxidation cycle

Abstract

Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the β-oxidation cycle by expressing the polyhydroxyalkanoate syntheses from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by using a mutant of the β-oxidation- associated multifunctional enzyme with low dehydrogenase activity toward R-3-hydroxybutyryl coenzyme A.