Microbiological Evaluation and Antibiotic Susceptibility Pattern of Bacterial Isolates Associated with Some Contaminated Edible Fruits and Vegetables

Abstract

Background: The present study was undertaken to study the percentage of pathogenic bacteria present in different fruit and vegetable samples available in the market in and around Kalaburagi, Karnataka, South India. Methods: A total of 940 different samples were collected from Kalaburagi, out of which 390 (23.84%) were fruits and 550 (35.27%) were vegetables. The pathogenic bacteria were isolated, by enrichment culture method using peptone water. The bacterial isolates were identified by convention microbial identification procedures. Result: Antibiotic resistant testing by disc diffusion method performed for E. coli, Salmonella and Shigella. Among the pathogens, E. coli (86.50%) of the isolates were resistant to Nalidixic acid while Imipenem and Trimethoprim-Sulfamethoxazole has the lowest resistance (19.84%), Salmonella (86.66%) isolates were resistant to Ciprofloxacin while Norfloxacin has the lowest resistance (4.76%) and Shigella (80.35%) isolates were resistant to Vancomycin while Amoxicillin has the lowest resistant (3.57%). Multiple drug resistance (MDR) was seen in E. coli at (38.88%), Salmonella at (26.66%) and Shigella at (10.71%) accordingly. The study therefore shown that E.coli, Salmonella and Shigella occur in Fruits and vegetables which collected from market place in Kalaburagi, Karnataka India, As per the results, adequate precaution should be taken while handles these fruits and vegetables. The antimicrobial resistance pattern shown by the isolates is an indication that adequate measurement needs to be taken to regulate the drug use in both humans and animals in order to minimize the risk of increasing antimicrobial resistance.