Direct comparison of heat-evoked activity of nociceptive neurons in the dorsal horn with the hindpaw withdrawal reflex in the rat

Abstract

Although the sensory coding of nociceptive neurons in the dorsal horn has been studied extensively, surprisingly little is known about how these neurons contribute to nociceptive reflexes. The objective of the present study was to examine the characteristics of dorsal horn neurons capable of initiating hindpaw withdrawal. To this end, neural and reflex activity were measured simultaneously in response to noxious radiant heat applied to the hindpaw in lightly anesthetized rats. Subsets of both multireceptive (MR; 52/95) and nociceptive-specific (NS; 19/46) neurons showed a consistent burst of activity that preceded the reflex. However, when compared with NS neurons, MR neurons as a group were: more likely to be active before the reflex (55 vs. 41%); more active before the reflex (31 vs. 23 Hz); and active earlier (2.8 vs. 2.3 s before the reflex). Subsets of MR neurons were active before the reflex regardless of receptive field size or location in the dorsal horn. In contrast, NS neurons with small receptive fields or those located outside of superficial laminae were rarely active before the reflex and thus unlikely to be part of the reflex circuit. These results suggest that current classification schemes, in particular MR and NS categories, cannot be used as the sole criterion to predict involvement in nociceptive reflexes. However, simultaneous measurement of neural and reflex activity provides an opportunity to determine the characteristics of nociceptive neurons involved in withdrawal reflexes.