Heterogenic serum, age, and multiplication fibroblasts

Abstract

The presence in a culture medium of heterogenic serum of various concentrations exerts a definite influence on the rate of multiplication of fibroblasts. Dog serum does not inhibit the growth of chicken fibroblasts markedly until its concentration reaches 15 per cent. Beyond this figure, each increase of the concentration brings about a rapid decrease in the rate of cell multiplication. Vv-hen the concentration reaches from 30 to 45 per cent, no growth takes place. The inhibiting action of cat serum begins to manifest itself at a concentration of 25 per cent and prevents cell proliferation completely at a concentration of 55 and 60 per cent. The ratio, Rate of growth in heterogenic serum/Rate of growth in chicken serum, can be taken as expressing the action of the serum on fibroblast multiplication; that is, as the growth index of the serum. © 1922, Rockefeller University Press., All rights reserved.