Isolation and Identification of Escherichia coli Producing Cytosine Deaminase from Iraqi patients

Abstract

This study was aimed to isolate a locally higher cytosine deaminase producer Escherichia coli. Ten E. coli bacterial isolates were obtained from twenty-five urine samples collected from Al-Imamein AlKadhumain medical city hospital in Baghdad. These isolates were identified by cultural, microscopical and biochemical tests, identifications also done by vitek 2 system. Cytosine deaminase activity was assayed for these isolates and it was found that all isolates were enzyme producer with variable activities and E. coli E9 was the most efficient with specific activity of (0.350 U/mg).