Endophytic bacteria (genus : Pseudomonas spp.) isolated from Aceh bamboo root as biological agent againts nematode Meloidogyne spp.

Abstract

Endophytic bacteria are bacteria that associated with plant tissues without causing any disease. Endophytic bacteria able to produce enzymes that act as nematicide against root knot disease in celery plants caused by Meloidogyne spp. Bamboo roots were isolated, obtain 26 isolate by screening of mortality test, and founded 14 isolates were potential as biological control agents with 100 percentage. Furthermore, 14 endophytic bacterial that has been isolates were detected their protease enzyme activity and 9 isolates were shown the protease enzyme as qualitatively result. Aplication of endophyte bacteria on Celery seedling detected that the treatment of bacteria (AB12) had the lowest number of gall, the lowest number of nematode populations in gall compared to other isolates. This result indicates the AB12 isolate similar characterize from genus Pseudomonas spp. have good potential as a biological control agent for Meloidogyne spp. in celery plants. Some endophytic bacteria in other treatments did not affect the inhibition of nematodes while were able to increase plant growth such as plant height, number of stems and higher root length (AB03 isolate).