Damidseq-pipeline: An automated pipeline for processing DamID sequencing datasets

Abstract

DamID is a powerful technique for identifying regions of the genome bound by a DNAbinding (or DNA-associated) protein. Currently, no method exists for automatically processing next-generation sequencing DamID (DamID-seq) data, and the use of DamID-seq datasets with normalization based on read-counts alone can lead to high background and the loss of bound signal. DamID-seq thus presents novel challenges in terms of normalization and background minimization. We describe here damidseq-pipeline, a software pipeline that performs automatic normalization and background reduction on multiple DamID-seq FASTQ datasets.