The isolation of Frankia from nodules of Casuarina

Abstract

Attempting to isolate Frankia from Casuarina, we found that this endophyte exhibited two unexpected characteristics: (i) an inability of most infected nodule cells to produce Frankia colonies; and (ii) the absence of specific nutritional requirements in the isolation medium. These findings led us to the formulation of some principles for isolating Frankia from Casuarina.