Isolation and in vitro Cultivation of an Unidentified Ciliate Causing Scuticociliatosis in Japanese Flounder (Paralichthys olivaceus).

Abstract

The ciliate causing scuticociliatosis in Japanese flounder was isolated, and axenic cultures were established. Sterilization of the ciliate was accomplished by serial washing with capillary pipettes. Growth of the ciliate was compared in different dilutions of Millport S solution containing 2% proteose peptone and 1% yeast extract at different temperatures. Optimum growth was obtained at dilution of 1:2 (osmolarity: 438 mosm) at 22.5-27.5-degrees-C with a maximum cell density of 1.0 X 10(4) cells/ml. PU - JAPAN SOC FISH PATHOL DEPT FISHERIES-FAC AGR PI - TOKYO PA - ACADEMY CENTER 358-5, YAMABUKI-CHO, SHINJUKU-KU, TOKYO, 162-0801, JAPAN